Device to remove dead cells from cultures generated using cryopreserved preparations.

Primary neuronal cultures revived from traditional cryopreserved preparations are notoriously contaminated with cells that perish during the freeze-thaw cycle. This lingering cellular debris severely limits the usability of these cultures in advanced imaging applications. More critically, it compromises drug toxicity and cell viability assays. When testing experimental compounds, researchers are left with a critical blind spot: it becomes nearly impossible to distinguish whether a cell was killed by the drug being tested, or if it was already dead prior to treatment.

To solve this, Spot Cells LLC has developed a proprietary device designed to selectively remove dead cells from revived cryopreserved cultures. The images above track the exact same microscopic field of murine primary cortical neurons over time. Our prototype device was applied on Day-In-Vitro 1 (DIV 1). Notice how the dead cells visible in the "before" image are completely cleared in the "after" image. Most importantly, the healthy neurons remain undisturbed, successfully extending their network of processes and continuing to thrive.

For inquiries, please contact spotcells@gmail.com.